|
KMID : 0855520070200020083
|
|
Korean Journal of Physical Anthropology
2007 Volume.20 No. 2 p.83 ~ p.92
|
|
|
Tissue Transglutaminase Activates NF-κB Pathway in the Atherosclerotic Coronary Artery
|
|
Kim Seung-Oh
Kim Min-Kyoung
Suh Dong-Hyun
Kim Chan-Woong
Jo Seung-Mook
Hahn Jang-Hee
Park Jeong-Hyun
Park Kyoung-Han
Kim Dae-Joong
|
|
|
Abstract
|
|
|
Molecular mechanism of nuclear factor-κB(NF-κB) in the atherosclerosis has been unclear. Recently, NF-κB activating function of tissue transglutaminase (tTGase), multifunctional calcium-dependent transamidation enzyme, have been reported in the various tissues like neuroglia.
In this report, we investigated the immunoreactivity of tTGase at the human atherosclerotic coronary artery, and examined the effect of tTGase on the well-known proatherogenic NF-κB pathway using tTGase-overexpressed cells.
Immunohistochemical studies on autopsy samples showed that immunoreactivity of tTGase was markedly elevated in the neointimal tissues of atherosclerotic coronary arteries with progression of disease. Immunohistochemical staining also demonstrated that phosphorylated I-κB was activated in the atherosclerotic vessel wall. In vitro study using rat cardiomyoblast (H9c2) and tTGase-overexpressed H9c2 showed that activated tTGase enhanced the phosphorylation of I-κB, and this activation was inhibited by tTGase specific inhibitors.
These findings suggest that cytosolic tTGase may serve as an activator of NF-κB.
|
|
|
KEYWORD
|
|
|
tissue transglutaminase, NF-κB, Atherosclerosis, Coronary artery
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|